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CC2
(Plasmid #21207)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21207 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pENTR1A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2200
  • Vector type
    Subclone vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Mek1
  • Alt name
    MAP2K1
  • Alt name
    ER-{alpha}
  • Alt name
    Esr1
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    2200
  • Mutation
    Mek1 R4F fused to ER*, constitutive active
  • Entrez Gene
    Esr1 (a.k.a. ER, ER-alpha, ERa, ERalpha, ESR, Estr, Estra, Nr3a1)
  • Entrez Gene
    MAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MKK1, PRKMK1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer pENTR-F
  • 3′ sequencing primer pENTR-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Constitutive active protein has the following aa sequence deleted - ALQKKLEELELDEQQRKRLE, residues 31 to 51.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CC2 was a gift from Paul Khavari (Addgene plasmid # 21207 ; http://n2t.net/addgene:21207 ; RRID:Addgene_21207)
  • For your References section:

    Mek1 alters epidermal growth and differentiation. Scholl FA, Dumesic PA, Khavari PA. Cancer Res. 2004 Sep 1;64(17):6035-40 10.1158/0008-5472.CAN-04-0017 PubMed 15342384