Addgene - GFP-C1(2)delta Plasmid Data

Plasmid 21216: GFP-C1(2)delta

Gene/insert name:
GFP-N2-PKCdelta-C1
Alt name:
PKCdelta
Insert size:
414
Species:
R. norvegicus (rat)
Entrez Gene:
Prkcd (Pkcd)
Fusion protein or tag:
GFP
Mutation:
C1(2) domains of PKC delta (rat) (Lab plasmid WC0001)
Vector backbone:
N2
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4596
Cloning site 5':
BglII
Site destroyed during cloning:
Unknown
Cloning site 3':
KpnI
Site destroyed during cloning:
Unknown
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Tobias Meyer
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Control of astrocyte Ca(2+) oscillations and waves by oscillating translocation and activation of protein kinase C. Codazzi et al (Curr Biol. 2001 Jul 24. 11(14):1089-97. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21216" in your Materials and Methods section.