PKC zeta WT
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21248||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namePKC zeta
SpeciesR. norvegicus (rat)
Insert Size (bp)1773
Entrez GenePrkcz (a.k.a. 14-3-3-zetaisoform, Pkcz, r14-3-3)
/ Fusion Protein
- HA (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PKC zeta WT was a gift from Bernard Weinstein (Addgene plasmid # 21248 ; http://n2t.net/addgene:21248 ; RRID:Addgene_21248)
For your References section:Roles of specific isoforms of protein kinase C in the transcriptional control of cyclin D1 and related genes. Soh JW, Weinstein IB. J Biol Chem. 2003 Sep 5. 278(36):34709-16. 10.1074/jbc.M302016200 PubMed 12794082