pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP
(Plasmid #213119)

• Purpose: Expression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cells
• Depositing Lab: Scott Coyle
• Publication: Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9. doi: 10.1016/j.cell.2023.12.007.

Backbone

• Vector backbone: pTwist SFFV
• Backbone manufacturer: Twist
• Backbone size w/o insert (bp): 5683
• Total vector size (bp): 8527
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ActA(1-584)-BFP-FKBP
• Species: Listeria monocytogenes
• Insert Size (bp): 2844
• Entrez Gene: actA (a.k.a. lmo0204)
• Promoter: SFFV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Purchased from Twist Bioscience

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry