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Plasmid 21318: PL-SIN-EOS-C(3+)-EGFP
  • Enhanced Green Fluorescence Protein

  • EGFP

  • 720

  • PL-SIN lentiviral vector
    (Search Vector Database)

  • Buzina et al., PLoS Genetics, 2008

  • Mammalian Expression, Lentiviral

  • 6531

  • EOS-C(3+) promoter region

  • Unknown

  • n/a List of Sequencing Primers

  • n/a

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • View sequences (2)
  • James Ellis

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

EGFP driven by EOS-C(3+) promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency. Hotta et al (Nat Methods. 2009 May . 6(5):370-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21318" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only