pHelper_AraC/pBad-Tnpase (camR)
(Plasmid
#213909)
-
PurposeArabinose-inducible himar1C9 transposase expression - cam resistance
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213909 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepSAM_AraC
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameAraC/pBad ATc-inducible himar1C9 transposase
-
SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
A portion of this plasmid was derived from a plasmid made byA portion of this plasmid was derived from pSAM_AraC (addgene #91569)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains an arabinose-inducible himar1C9 transposase. The backbone encodes for chloramphenicol resistance on a medium copy p15A backbone.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHelper_AraC/pBad-Tnpase (camR) was a gift from James Collins (Addgene plasmid # 213909 ; http://n2t.net/addgene:213909 ; RRID:Addgene_213909) -
For your References section:
A self-propagating, barcoded transposon system for the dynamic rewiring of genomic networks. English MA, Alcantar MA, Collins JJ. Mol Syst Biol. 2023 Jun 12;19(6):e11398. doi: 10.15252/msb.202211398. Epub 2023 Mar 27. 10.15252/msb.202211398 PubMed 36970845
