pT7-PE7 for IVT
(Plasmid #214813)

• Purpose: Template for in vitro transcription of PE7
• Depositing Lab: Brittany Adamson
• Publication: Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-024-07259-6.

Backbone

• Vector backbone: pT7
• Backbone size w/o insert (bp): 3481
• Total vector size (bp): 10552
• Modifications to backbone: Inactivated T7 promoter to prevent transcription from plasmid
• Vector type: Template for in vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PE7
• Alt name: PEmax-La(SSB)1-194
• Species: Synthetic; SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus; La(SSB)1-194 is from Homo sapiens
• Insert Size (bp): 7071
• Promoter: T7 (inactivated)
• Tags / Fusion Proteins:
  • SV40 bpNLS (N terminal on insert)
  • SV40 bpNLS (C terminal on insert)
  • c-Myc NLS (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: M13 Reverse

Resource Information

• Supplemental Documents:
  • pT7-PE7 for IVT.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry