Addgene - pMBP-HIS2-Snf7 Plasmid Data

Plasmid 21492: pMBP-HIS2-Snf7

Gene/insert name:
SNF7
Alt name:
DID1
Alt name:
VPS32
Insert size:
720
Species:
S. cerevisiae (budding yeast)
Entrez Gene:
SNF7 (DID1, RNS4, VPL5, VPS32)
Fusion protein or tag:
6x His
Terminal:
N terminal on backbone
Fusion protein or tag:
MBP
Terminal:
N terminal on backbone
Mutation:
none
Vector backbone:
pHis2
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5549
Cloning site 5':
N/A (RF cloning used)
Site destroyed during cloning:
No
5' sequencing primer:
T7 promotor List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
James Hurley
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Membrane scission by the ESCRT-III complex. Wollert et al (Nature. 2009 Mar 12. 458(7235):172-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21492" in your Materials and Methods section.