pMSCV Cyclin D2-short
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21655||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6295
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCyclin D2
SpeciesH. sapiens (human)
Insert Size (bp)1200
Entrez GeneCCND2 (a.k.a. KIAK0002, MPPH3)
- Cloning method Restriction Enzyme
- 5′ cloning site Bgl II (not destroyed)
- 3′ cloning site Hpa I (not destroyed)
- 5′ sequencing primer pBABE 5
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
Terms and Licenses
Short means always shortest 3’UTR due to alternative polyadenylation.
Constructs for cell cycle
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSCV Cyclin D2-short was a gift from David Bartel (Addgene plasmid # 21655 ; http://n2t.net/addgene:21655 ; RRID:Addgene_21655)
For your References section:Widespread shortening of 3'UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells. Mayr C, Bartel DP. Cell. 2009 Aug 21. 138(4):673-84. 10.1016/j.cell.2009.06.016 PubMed 19703394