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pMSCV PIG IMP-1-long-mut
(Plasmid #21661)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21661 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMSCV PIG
  • Backbone size w/o insert (bp) 7600
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IGF-II mRNA-binding protein 1
  • Alt name
    IMP-1
  • Alt name
    CM20
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8500
  • Mutation
    mutated let-7 binding sites.
  • Entrez Gene
    IGF2BP1 (a.k.a. CRD-BP, CRDBP, IMP-1, IMP1, VICKZ1, ZBP1)
  • Tags / Fusion Proteins
    • IRES (C terminal on backbone)
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer pBABE 5
  • 3′ sequencing primer MSCV-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Long means that all functional alternative polyadenylation signals have been deleted.

Mut means that all microRNA binding sites for an indicated microRNA have been mutated.

Constructs for Soft-agar assay

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV PIG IMP-1-long-mut was a gift from David Bartel (Addgene plasmid # 21661 ; http://n2t.net/addgene:21661 ; RRID:Addgene_21661)
  • For your References section:

    Widespread shortening of 3'UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells. Mayr C, Bartel DP. Cell. 2009 Aug 21. 138(4):673-84. 10.1016/j.cell.2009.06.016 PubMed 19703394