pFP140
(Plasmid #21672)

• Depositing Lab: James Haber
• Publication: Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71.

Backbone

• Vector backbone: Ted
• Backbone manufacturer: W. Kramer
• Vector type: centromeric plasmid
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Lacz
• Alt name: 2 copies of lacZ
• Alt name: pJH1442
• Alt name: see author's map
• Species: E. coli
• Insert Size (bp): 3000
• Mutation: The second lacZ sequence has been modified, the EcoRV/BclI fragment has been deleted. Second LacZ HO site is no longer present.
• Entrez Gene: lacZ (a.k.a. ECIAI39_0334)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bam Hi (not destroyed)
• 3′ cloning site: Eco RI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • Ted correspondence
  • Ted Map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Derived from Ted, a centromeric plasmid (provided by W. Kramer) marked by the URA3 gene. Contains two copies of the Escherichia coli lacZ
gene in inverted orientation, with one copy containing an HO endonuclease
cleavage site.

Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.