pJF6
(Plasmid #21678)

• Depositing Lab: James Haber
• Publication: Fishman-Lobell et al Mol Cell Biol. 1992 Mar . 12(3):1292-303.

Backbone

• Vector backbone: pJH271
• Vector type: centromeric plasmid
• Selectable markers: LEU2, URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LacZ
• Alt name: 2 direct repeat .copies of lacZ
• Alt name: see author's map
• Species: E. coli
• Mutation: HO digestion site in one of the LacZ genes
• Entrez Gene: lacZ (a.k.a. ECIAI39_0334)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Hind III (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The HindIII fragment of pJF3 containing URA3 UAScycl- and 300 bp of the LEU2 gene promoter and
LacZ::CS cut site was inserted into the HindIII site at the
upstream end of a promotorless LacZ gene on plasmid JH271.

The two LacZ sequences are in direct repeat orientation relative to each other.

The lambda sequences are of unknown origin

Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.