|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21730||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBlueScript II KS(+)
- Backbone size w/o insert (bp) 5600
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert namep21 promoter deletion mutant
Alt namep21, WAF1
SpeciesH. sapiens (human)
Insert Size (bp)1447
MutationIdentical to 2-Luc except that the 50-bp region between -144 and -95 has been deleted.
Entrez GeneCDKN1A (a.k.a. CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:2-Luc-Del-50 was a gift from Wafik El-Deiry (Addgene plasmid # 21730 ; http://n2t.net/addgene:21730 ; RRID:Addgene_21730)
For your References section:AP2 inhibits cancer cell growth and activates p21WAF1/CIP1 expression. Zeng YX, Somasundaram K, el-Deiry WS. Nat Genet. 1997 Jan . 15(1):78-82. 10.1038/ng0197-78 PubMed 8988173