pCL1271
(Plasmid #217963)

• Purpose: Mammalian expression of Importin 5 (Imp5, IPO5) with an N-terminal eGFP fusion. Splice isoform 1.
• Depositing Lab: Andrew Bowman
• Publication: Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 10.15252/embj.201798714. Epub 2018 Sep 3.

Backbone

• Vector backbone: peGFP-C1
• Backbone manufacturer: Clonetech
• Vector type: Mammalian Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Imp5
• Alt name: Importin 5
• Species: H. sapiens (human)
• Entrez Gene: IPO5 (a.k.a. IMB3, KPNB3, Pse1, RANBP5, imp5)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: pEGFP-C2 FP
• 3′ sequencing primer: SV40-RP

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry