pAAV-hSyn-FLEX-H2BmTagBFP2-oG
(Plasmid
#217976)
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PurposeAAV transfer plasmid to express H2B-BFP and codon-optimized Rabies G protein
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 217976 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
This item is currently unavailable outside the US without additional regulatory approval.
A non-refundable shipping export licensing fee of $85 is required to cover Addgene’s additional processing costs.
Backbone
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Vector backbonepAAV
- Total vector size (bp) 7275
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameH2BmTagBFP2
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Alt nameHistone2B-BFP
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SpeciesSynthetic
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Insert Size (bp)1107
- Promoter hSyn
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Tag
/ Fusion Protein
- mTAGBFP2 (C terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer pBR322ori-F (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameoG
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Alt nameCodon-optimized Rabies G protein
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SpeciesSynthetic
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Insert Size (bp)1575
- Promoter hSyn
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer pBR322ori-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn-FLEX-H2BmTagBFP2-oG was a gift from Edward Callaway (Addgene plasmid # 217976 ; http://n2t.net/addgene:217976 ; RRID:Addgene_217976) -
For your References section:
Postsynaptic cell type and synaptic distance do not determine efficiency of monosynaptic rabies virus spread measured at synaptic resolution. Patino M, Lagos WN, Patne NS, Miyazaki PA, Bhamidipati SK, Collman F, Callaway EM. Elife. 2023 Dec 14;12:e89297. doi: 10.7554/eLife.89297. 10.7554/eLife.89297 PubMed 38096019
Map uploaded by the depositor.
