pSAD-5PSD95-EGFP-SynPhRFP
(Plasmid
#217979)
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PurposeG-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFP
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 217979 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
This item is currently unavailable outside the US without additional regulatory approval.
A non-refundable shipping export licensing fee of $85 is required to cover Addgene’s additional processing costs.
Backbone
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Vector backbonepSAD
- Total vector size (bp) 18491
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Vector typeG-Deleted Rabies
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert name5PSD95-EGFP
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Alt namePostsynaptic Density Protein-EGFP
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SpeciesR. norvegicus (rat)
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Insert Size (bp)2946
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Entrez GeneDlg4 (a.k.a. Dlgh4, PSD95, Sap90)
- Promoter None
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameSynPhRFP
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Alt nameSynaptophysin-RFP
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SpeciesM. musculus (mouse)
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Insert Size (bp)1695
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Entrez GeneSyp (a.k.a. A230093K24Rik, Syn, p38)
- Promoter None
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Tag
/ Fusion Protein
- RFP (C terminal on insert)
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSAD-5PSD95-EGFP-SynPhRFP was a gift from Edward Callaway (Addgene plasmid # 217979 ; http://n2t.net/addgene:217979 ; RRID:Addgene_217979) -
For your References section:
Postsynaptic cell type and synaptic distance do not determine efficiency of monosynaptic rabies virus spread measured at synaptic resolution. Patino M, Lagos WN, Patne NS, Miyazaki PA, Bhamidipati SK, Collman F, Callaway EM. Elife. 2023 Dec 14;12:e89297. doi: 10.7554/eLife.89297. 10.7554/eLife.89297 PubMed 38096019
Map uploaded by the depositor.
