AIRAP 11: PSMD14.pEYFP.V1
(Plasmid #21801)

• Depositing Lab: David Ron
• Publication: Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Backbone

• Vector backbone: pEYFP
• Backbone manufacturer: Clontech (?)
• Backbone size w/o insert (bp): 4200
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PSMD14
• Species: M. musculus (mouse)
• Entrez Gene: Psmd14 (a.k.a. Pad1, Poh1, rpm11, AA986732, 2610312C03Rik, 3200001M20Rik)
• Tag / Fusion Protein:
  • YFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that though there is a Kan resistance gene in this plasmid according to the author's sequence, the gene is not functional. This plasmid does NOT confer Kan resistance.