pLenti-Puro 2xStrep-HPC4-Gb5
(Plasmid
#218177)
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PurposeUsed to make a lenti virus for stable expression of the G protein beta 5 subunit in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 218177 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLenti puro
- Total vector size (bp) 8305
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameG beta subunit 5
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SpeciesH. sapiens (human)
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Entrez GeneGNB5 (a.k.a. GB5, HG2E, IDDCA, LADCI, LDMLS1, LDMLS2, gbeta5)
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Tags
/ Fusion Proteins
- 2x Strep tag (N terminal on insert)
- HPC4 (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-Puro 2xStrep-HPC4-Gb5 was a gift from Barry Willardson (Addgene plasmid # 218177 ; http://n2t.net/addgene:218177 ; RRID:Addgene_218177) -
For your References section:
Visualizing the chaperone-mediated folding trajectory of the G protein beta5 beta-propeller. Wang S, Sass MI, Kwon Y, Ludlam WG, Smith TM, Carter EJ, Gladden NE, Riggi M, Iwasa JH, Willardson BM, Shen PS. Mol Cell. 2023 Nov 2;83(21):3852-3868.e6. doi: 10.1016/j.molcel.2023.09.032. Epub 2023 Oct 17. 10.1016/j.molcel.2023.09.032 PubMed 37852256
Map uploaded by the depositor.
