ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG-luc
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21850||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5010
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
SpeciesM. musculus (mouse)
Insert Size (bp)101
MutationATF4 5' UTR and AUG fused to Luciferase driven by the TK promoter
Entrez GeneAtf4 (a.k.a. Atf-, Atf-4, C/AT, C/ATF, CREB, CREB-2, CREB2, TAXREB, TAXREB67)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
Addgene sequencing results show 2 mismatches with author's sequence and NCBI sequence for ATF4.
Mouse ATF4 5' UTR and AUG fused to Luciferase driven by the TK promoter in a pGL3 backbone
TK.mATF4.UTR.Luc.pGL3 (5202nt, predicted). TK promoter: 58..184; mATF4 5'UTR and AUG: 185..470; luciferase coding sequence: 472..2121; poly A signals: 2156..2377
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG-luc was a gift from David Ron (Addgene plasmid # 21850 ; http://n2t.net/addgene:21850 ; RRID:Addgene_21850)
For your References section:Regulated translation initiation controls stress-induced gene expression in mammalian cells. Harding HP, Novoa I, Zhang Y, Zeng H, Wek R, Schapira M, Ron D. Mol Cell. 2000 Nov . 6(5):1099-108. 10.1016/S1097-2765(00)00108-8 PubMed 11106749