Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

TrHKI-pGFPN3
(Plasmid #21918)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21918 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGF-N3
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha, HB101 and other general purpose strains
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Truncated human HKI (lacking exon 1 sequence that encodes for the mitochondrial binding domain) in pGFP-N3 plasmid
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2688
  • Mutation
    This is the truncated human HKI cDNA (lacking exon 1 that encodes for the mitochondrial binding domain of HKI) in pGFP-N3
  • GenBank ID
    M75126
  • Entrez Gene
    HK1 (a.k.a. HK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, RP79, hexokinase)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TrHKI-pGFPN3 was a gift from Hossein Ardehali (Addgene plasmid # 21918 ; http://n2t.net/addgene:21918 ; RRID:Addgene_21918)
  • For your References section:

    Glucose phosphorylation and mitochondrial binding are required for the protective effects of hexokinases I and II. Sun L, Shukair S, Naik TJ, Moazed F, Ardehali H. Mol Cell Biol. 2008 Feb . 28(3):1007-17. 10.1128/MCB.00224-07 PubMed 18039843