1
Plasmid 21978: U6+27-20pf
  • U6 miR-20 perfect

  • 50

  • U6+27
    (Search Vector Database)

  • Mammalian Expression

  • 5000

  • XhoI

  • No

  • ApaI

  • No

  • n/a List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • Phil Sharp

  • UBMTA

Comments: 

miR-20 perfect CMV sponge and U6 sponge, 2 sites:

CUACCUGCACUAUAAGCACUUUA

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Ebert et al (Nat Methods. 2007 Sep . 4(9):721-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21978" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with