Addgene - U6+27-20pf Plasmid Data

Plasmid 21978: U6+27-20pf

Gene/insert name:
U6 miR-20 perfect
Insert size:
50
Vector backbone:
U6+27
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5000
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
ApaI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Phil Sharp
Terms and Licenses:
MTA

Comments:

miR-20 perfect CMV sponge and U6 sponge, 2 sites:

CUACCUGCACUAUAAGCACUUUA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Ebert et al (Nat Methods. 2007 Sep . 4(9):721-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21978" in your Materials and Methods section.