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Browse > Chang-Deng Hu > Shyu et al > pBiFC-VN173
Plasmid 22010: pBiFC-VN173

  • VN173

  • Venus (1-172)

  • 516

  • Aequorea victoria

  • FLAG

  • N terminal on backbone

  • Venus residus 1-172

  • pFlag-CMV
    (Search Vector Database)

  • Sigma

  • Mammalian Expression

  • 4700

  • Xba I

  • No

  • BamHI

  • No

  • CMV-F List of Sequencing Primers

  • hGH-PA-R

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)

  • Chang-Deng Hu

  • MTA

Comments: 

Shyu Y, Suarez C, and Hu, CD. Visualization of ternary complexes in living cells by using a BiFC-based FRET analysis. Nature Protocols, 3:1693-1702 (2008)

Multicloning sites are available between FLAG and VN173

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. Shyu et al (Biotechniques. 2006 Jan . 40(1):61-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22010" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only