Addgene - pTriEx-mVenus-PA-Rac1-T17N Plasmid Data

Plasmid 22017: pTriEx-mVenus-PA-Rac1-T17N

Gene/insert name:
PA-Rac1-T17N
Alt name:
LOV(Leu404-Leu546)-Rac1(Ile4)-T17N
Insert size:
1100
Species:
H. sapiens (human)
Species:
Avena sativa (oat)
Fusion protein or tag:
6X His
Terminal:
N terminal on backbone
Fusion protein or tag:
mVenus
Terminal:
N terminal on backbone
Mutation:
Rac1 starts at I4 and contains mutation T17N.
Vector backbone:
pTriEx
(Search Vector Database)
Backbone manufacturer:
Novagen
Vector type:
Mammalian Expression, Bacterial Expression, Insect Expression
Backbone size w/o insert (bp):
6000
Cloning site 5':
Bam HI
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
pTriExUP List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Klaus Hahn
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pTriEx Assurance Letter

Comments:

Protocols for production and use of Hahn lab biosensors and photoactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments!

Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html

Please note, Addgene has not confirmed all the mutations associated with this construct. We suggest sequence verifying this plasmid upon receipt.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A genetically encoded photoactivatable Rac controls the motility of living cells. Wu et al (Nature. 2009 Sep 3. 461(7260):104-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22017" in your Materials and Methods section.