|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22025||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6000
Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameLOV(Leu404-Leu546 , C450A)-Rac1(Ile4)-Q61L/E91H/N92H
SpeciesH. sapiens (human); Avena sativa (oat)
Insert Size (bp)1100
MutationLOV domain contains mutation C450A.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H.
Entrez GeneRAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
/ Fusion Protein
- 6X His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pTriExUP (Common Sequencing Primers)
Protocols for production and use of Hahn lab biosensors and photoactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments!
Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html
Please note, Addgene has not confirmed all the mutations associated with this construct. We suggest sequence verifying this plasmid upon receipt.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTriEx-PA-Rac1-C450A was a gift from Klaus Hahn (Addgene plasmid # 22025 ; http://n2t.net/addgene:22025 ; RRID:Addgene_22025)
For your References section:A genetically encoded photoactivatable Rac controls the motility of living cells. Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn KM. Nature. 2009 Sep 3. 461(7260):104-8. 10.1038/nature08241 PubMed 19693014