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Plasmid 22025: pTriEx-PA-Rac1-C450A
  • PA-Rac1-C450A

  • LOV(Leu404-Leu546 , C450A)-Rac1(Ile4)-Q61L/E91H/N92H

  • 1100

  • H. sapiens (human); Avena sativa (oat)

  • 6X His

  • N terminal on backbone

  • LOV domain contains mutation C450A. Rac1 starts at I4 and contains mutations Q61L, E91H and N92H.

  • pTriEx
    (Search Vector Database)

  • Novagen

  • Mammalian Expression, Bacterial Expression, Insect Expression

  • 6000

  • Bam HI

  • No

  • HindIII

  • No

  • pTriExUP List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • Klaus Hahn

  • UBMTA
    EMD Millipore Limited Use Label License/Brookhaven pTriEx Assurance Letter

Comments: 

Protocols for production and use of Hahn lab biosensors and photoactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments!

Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html

Please note, Addgene has not confirmed all the mutations associated with this construct. We suggest sequence verifying this plasmid upon receipt.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A genetically encoded photoactivatable Rac controls the motility of living cells. Wu et al (Nature. 2009 Sep 3. 461(7260):104-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22025" in your Materials and Methods section.