Addgene - pHTIKA Plasmid Data

Plasmid 22062: pHTIKA

Gene/insert name:
hCMV-TetOn expression cassette
Alt name:
hCMV-rTTA-IRES-tsKID
Insert size:
3423
Vector backbone:
pBS
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
2389
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
BglII
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Maria Castro
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Regulatable gutless adenovirus vectors sustain inducible transgene expression in the brain in the presence of an immune response against adenoviruses. Xiong et al (J Virol. 2006 Jan . 80(1):27-37. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22062" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

22061: pMTIKA

19766: pLV-tetO-Oct4

19763: pLV-tetO-myc T58A

19765: pLV-tetO-Sox2

19764: pLV-tetO-Klf4