AAVS1 hPGK-PuroR-pA donor
(Plasmid #22072)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    N/A
  • Backbone size w/o insert (bp) 5876
  • Vector type
    ZFN donor
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    PGK-Puro
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1105

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1 hPGK-PuroR-pA donor was a gift from Rudolf Jaenisch (Addgene plasmid # 22072)
  • For your References section:

    Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R. Nat Biotechnol. 2009 Aug 13. ():. 10.1038/nbt.1562 PubMed 19680244