Addgene - AAVS1 hPGK-PuroR-pA donor Plasmid Data

Plasmid 22072: AAVS1 hPGK-PuroR-pA donor

Gene/insert name:
PGK-Puro
Insert size:
1105
Species:
H. sapiens (human)
Vector backbone:
N/A
(Search Vector Database)
Vector type:
ZFN donor
Backbone size w/o insert (bp):
5876
5' sequencing primer:
N/A List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Rudolf Jaenisch
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Hockemeyer et al (Nat Biotechnol. 2009 Aug 13. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22072" in your Materials and Methods section.