mBEN 1957
(Plasmid #22203)

• Depositing Lab: Ananda Roy
• Publication: Lazebnik et al J Biol Chem. 2008 Apr 25. 283(17):11078-82.

Backbone

• Vector backbone: pLL3.7
• Backbone size w/o insert (bp): 7650
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stable 3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Alt name: BEN
• Insert Size (bp): 55
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: U6
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The hairpin sequence is
5'TGC GAG CAA CAG CAT CCA GTT TCA AGA GAA CTG GAT GCT GTT GCT CGC TTT TTT C 3'

May contain a GFP cassette or a puro cassette.