Addgene - pLenti CMV/TO p16 Hygro (w184-1) Plasmid Data

Plasmid 22263: pLenti CMV/TO p16 Hygro (w184-1)

Gene/insert name:
cyclin-dependent kinase inhibitor 2A
Alt name:
p16
Insert size:
446
Species:
H. sapiens (human)
Entrez Gene:
CDKN2A (ARF, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16)
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
8722
Cloning site 5':
Bam HI
Site destroyed during cloning:
No
Cloning site 3':
Xba I
Site destroyed during cloning:
No
5' sequencing primer:
CMVforw List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3, 370C
High or low copy:
High Copy
Selectable markers:
Hygromycin
Person or lab that originally
cloned the gene/insert:
p16 cDNA was obtained from Dr. Christian Beausejour.
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22263" in your Materials and Methods section.