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Plasmid 22273: pQCXIH CMV/TO SV40 Large T K1 mutant (w417-1)
  • SV40 Large T antigen K1 mutant

  • Large T K1

  • 2126

  • SV40gp6 (SV40gp6)

  • E107K (K1 mutation)

  • pQCXIH
    (Search Vector Database)

  • Clontech

  • Mammalian Expression, Retroviral

  • 7911

  • Bam HI

  • No

  • Xba I

  • No

  • CMVforw List of Sequencing Primers

  • n/a

  • Ampicillin

  • DH5alpha

  • 37

  • TOP10F', 37oC

  • High Copy

  • Hygromycin

  • Large T K1 mutant was obtained from Dr. Francis Rodier.

  • View sequences (3)
  • View map

  • Eric Campeau

  • UBMTA

Comments: 

The Large T antigen K1 mutant can no longer bind and inactivate the Rb, p130 and p107 proteins (DeCaprio et al. 1988, Stubdal et al. 1997). It also fails to activate Akt (Yu and Alwine 2008).

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22273" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only