pQCXIH CMV/TO SV40 Large T K1 mutant (w417-1)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22273||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7911
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Growth instructionsTOP10F', 37oC
Copy numberHigh Copy
Gene/Insert nameSV40 Large T antigen K1 mutant
Alt nameLarge T K1
Insert Size (bp)2126
MutationE107K (K1 mutation)
Entrez GeneSV40gp6 (a.k.a. SV40gp6)
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer CMVforw
- 3′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byLarge T K1 mutant was obtained from Dr. Francis Rodier.
Terms and Licenses
The Large T antigen K1 mutant can no longer bind and inactivate the Rb, p130 and p107 proteins (DeCaprio et al. 1988, Stubdal et al. 1997). It also fails to activate Akt (Yu and Alwine 2008).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQCXIH CMV/TO SV40 Large T K1 mutant (w417-1) was a gift from Eric Campeau (Addgene plasmid # 22273)