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pAL204
(Plasmid #22282)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22282 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT
  • Alt name
    phyB
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    3551
  • Entrez Gene
    PHYB (a.k.a. AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Kras4B [Entrez Gene ID: 3845] polybasic CaaX terminus
(KKKKKKSKTKCVIM) [ggt aaa aag aag aaa aag aag tca aag aca aag tgt gta att atg].

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAL204 was a gift from Christopher Voigt (Addgene plasmid # 22282)
  • For your References section:

    Spatiotemporal control of cell signalling using a light-switchable protein interaction. Levskaya A, Weiner OD, Lim WA, Voigt CA. Nature. 2009 Sep 13. ():. 10.1038/nature08446 PubMed 19749742