|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22306||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5857
Vector typeYeast Expression ; 2micron
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer GAL1
- 3′ sequencing primer CYC1 (Common Sequencing Primers)
Terms and Licenses
Galactose-inducible expression plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYES2.1-Ago1 was a gift from David Bartel (Addgene plasmid # 22306 ; http://n2t.net/addgene:22306 ; RRID:Addgene_22306)
For your References section:RNAi in Budding Yeast. Drinnenberg IA, Weinberg DE, Xie KT, Mower JP, Wolfe KH, Fink GR, Bartel DP. Science. 2009 Sep 10. ():. 10.1126/science.1176945 PubMed 19745116