pTag4C_NLGN1-CTEV-2xHA-PBM(TTRV)
(Plasmid
#223306)
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Purposeconstitutive expression of NLGN1 - CTEV fusion with Nlgn1 PDZ binding motif extracted on the C-terminal end
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 223306 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepTag4C
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNlgn1 - CTEV - PBM (TTRV)
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SpeciesM. musculus (mouse)
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Entrez GeneNlgn1 (a.k.a. 6330415N05Rik, NL1, Nlg1, mKIAA1070)
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Tags
/ Fusion Proteins
- FLAG (C terminal on backbone)
- 2xHA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTag4C_NLGN1-CTEV-2xHA-PBM(TTRV) was a gift from Michael Wehr (Addgene plasmid # 223306)