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pBABEpuro GFP-LC3
(Plasmid #22405)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22405 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBABE-puro
  • Backbone manufacturer
    Addgene plasmid # 1764
  • Backbone size w/o insert (bp) 5169
  • Modifications to backbone
    GFP-LC3 was subcloned from pEGFPC1-LC3 into the SnaB1 and Sal1 sites of pBABEpuro
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    microtubule-associated protein 1 light chain 3 beta
  • Alt name
    Map1lc3b
  • Alt name
    LC3b
  • Alt name
    LC3
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1500
  • GenBank ID
    U05784
  • Entrez Gene
    Map1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE-5
  • 3′ sequencing primer pBABE-3
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. Kabeya Y et al. (EMBO J. 2000 Nov 1. 19(21):5720-8.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABEpuro GFP-LC3 was a gift from Jayanta Debnath (Addgene plasmid # 22405 ; http://n2t.net/addgene:22405 ; RRID:Addgene_22405)
  • For your References section:

    Induction of autophagy during extracellular matrix detachment promotes cell survival. Fung C, Lock R, Gao S, Salas E, Debnath J. Mol Biol Cell. 2008 Mar . 19(3):797-806. 10.1091/mbc.e07-10-1092 PubMed 18094039