Addgene - pBABE-puro mCherry-EGFP-LC3B Plasmid Data

Plasmid 22418: pBABE-puro mCherry-EGFP-LC3B

Gene/insert name:
microtubule-associated protein 1 light chain 3 beta
Alt name:
MAP1LC3B
Alt name:
LC3B
Alt name:
LC3
Insert size:
1900
Species:
H. sapiens (human)
GenBank ID:
BC045759
Entrez Gene:
MAP1LC3B (ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
Fusion protein or tag:
mCherry
Terminal:
N terminal on insert
Fusion protein or tag:
eGFP
Terminal:
N terminal on insert
Vector backbone:
pBABE-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
BamHI
Site destroyed during cloning:
Yes
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
pDest-mCherry-EGFP-hLC3b from Terje Johansen from the Biochemistry Department, Institute of Medical Biology, University of Tromsøl.
Sequence: View sequences (3)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Jayanta Debnath
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: PLIC proteins or ubiquilins regulate autophagy-dependent cell survival during nutrient starvation. N'Diaye et al (EMBO Rep. 2009 Feb;10(2):173-9. doi: 10.1038/embor.2008.238. Epub 2009 Jan 16. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22418" in your Materials and Methods section.