Addgene - pSicoR (Puro) NEMO shRNA2 Plasmid Data

Plasmid 22506: pSicoR (Puro) NEMO shRNA2

Gene/insert name:
NEMO shRNA2
Alt name:
NEMO
Species:
M. musculus (mouse)
Entrez Gene:
Ikbkg (RP23-15J3.1, 1110037D23Rik, AI848108, AI851264, AW124339, IKK[g], NEMO)
Vector backbone:
pSicoR PGK puro
(Search Vector Database)
Backbone manufacturer:
Available at Addgene (#12084)
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size w/o insert (bp):
7700
5' sequencing primer:
mU6-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

Mouse NEMO shRNA2 in pSicoReverse (Puromycin)
forward oligo:
TGGACAAGGCCTCTGTGAAATTCAAGAGATTTCACAGAGGCCTTGTCCTTTTTTC
reverse oligo :
TCGAGAAAAAAGGACAAGGCCTCTGTGAAATCTCTTGAATTTCACAGAGGCCTTGTCCA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22506" in your Materials and Methods section.