p65.pA.NGFR.mCMV.hPGK.mOva.wpre.dna
(Plasmid
#225216)
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PurposeOVA-pdgfr(TM) in p1074; express a membrane-bound ovalbumin(mOVA).
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 225216 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonep1074.NGFR.mCMV.hPGK.GFP.wpre
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Backbone manufacturerNaldini lab
- Backbone size w/o insert (bp) 9213
- Total vector size (bp) 9848
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOVA-pdgfr
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Alt nameovalbumin (chicken)
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SpeciesG. gallus (chicken)
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Insert Size (bp)1445
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Entrez GeneOVAL (a.k.a. OVA, SERPINB14)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BlpI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer add
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://ssrn.com/abstract=4912248 for preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p65.pA.NGFR.mCMV.hPGK.mOva.wpre.dna was a gift from Ferdinando Pucci (Addgene plasmid # 225216)