Addgene - pLenti X1 Zeo/pTER shGFP #2 (733-1) Plasmid Data

Plasmid 22531: pLenti X1 Zeo/pTER shGFP #2 (733-1)

Gene/insert name:
sh Enhanced Green Fluorescent Protein
Alt name:
shGFP
Insert size:
48
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Lentiviral, RNAi
Backbone size w/o insert (bp):
5541
Cloning site 5':
Bgl II
Site destroyed during cloning:
Yes
Cloning site 3':
Hind III
Site destroyed during cloning:
No
5' sequencing primer:
H1forw List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Bleocin (Zeocin)
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3, 37oC
High or low copy:
High Copy
Selectable markers:
Zeocin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22531" in your Materials and Methods section.