Addgene - pKO2.2 Plasmid Data

Plasmid 22676: pKO2.2

Gene/insert name:
DTA
Insert size:
500
Mutation:
pDTA for targeting vector generation. I-CeuI linearization site.
Vector backbone:
pKO2
(Search Vector Database)
Vector type:
Mouse Targeting
Backbone size w/o insert (bp):
3000
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Robert Benezra
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: High levels of Id1 expression define B1 type adult neural stem cells. Nam et al (Cell Stem Cell. 2009 Nov 6. 5(5):515-26. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22676" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

22209: OCT4 GFP puro Donor 1