pB6-ROSA26::lacZ
(Plasmid #22678)

• Depositing Lab: Robert Benezra
• Publication: Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Backbone

• Vector backbone: SAbetageolox2DTA
• Backbone size w/o insert (bp): 9000
• Vector type: Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: beta-geo
• Insert Size (bp): 3000
• Mutation: Targeting vector for the Gt(ROSA26) locus. Beta-geo insert.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Upon NGS sequencing, we discovered that an incorrect clone had been deposited. The author of this paper is no longer in the laboratory, and we do not have the clones to re-deposit. We note that this plasmid is a construct distinct from the ROSA26-CAG-lsl-tauLacZ reporter construct described in Nam and Benezra, 2009. If the user seeks B6J ROSA26 locus homology arms for targeting vector construction, we recommend plasmids deposited at http://www.addgene.org/browse/article/28203395/