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pBT-3
(Plasmid #22832)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer tggtcagtat tgagcgatat
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Addgene's QC sequence shows a 3bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBT-3 was a gift from Ryan Gill (Addgene plasmid # 22832)
  • For your References section:

    Broad host range vectors for stable genomic library construction. Lynch MD, Gill RT. Biotechnol Bioeng. 2006 May 5. 94(1):151-8. 10.1002/bit.20836 PubMed 16496398