|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22927||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression, AAV
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameperoxisome proliferator activated receptor gamma binding site promoter with neuron-restrictive silencer element with minimal CMV
Alt namePparg DR1
Insert Size (bp)1700
- Cloning method Restriction Enzyme
- 5′ sequencing primer Unknown
- 3′ sequencing primer SP6 (Common Sequencing Primers)
There is a gap in alignment between depositor's sequence
and Addgene's sequence. The gap is probably part of the cloning site. Also, this promoter sequence
is largely artificial, and won't show by BLAST.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-DR1-NRSE-RFP was a gift from Edward Callaway (Addgene plasmid # 22927 ; http://n2t.net/addgene:22927 ; RRID:Addgene_22927)
For your References section:Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S, Callaway EM. Front Neural Circuits. 2009 . 3():19. 10.3389/neuro.04.019.2009 PubMed 19949461