Addgene - pLenti6/V5-p53_R280K Plasmid Data

Plasmid 22933: pLenti6/V5-p53_R280K

Gene/insert name:
tumor protein 53
Alt name:
TP53
Insert size:
1179
Species:
H. sapiens (human)
Entrez Gene:
TP53 (BCC7, LFS1, P53, TRP53)
Fusion protein or tag:
v5
Terminal:
C terminal on backbone
Mutation:
R280K mutant
Vector backbone:
pLenti6/V5-D-Topo
(Search Vector Database)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
6963
Cloning method:
TOPO Cloning
5' sequencing primer:
CMV List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3 (Invitrogen)
High or low copy:
Unknown
Selectable markers:
Blasticidin
Sequence: View sequences (2)
Principal Investigator:
Bernard Futscher
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Different mutant/wild-type p53 combinations cause a spectrum of increased invasive potential in nonmalignant immortalized human mammary epithelial cells. Junk et al (Neoplasia. 2008 May . 10(5):450-61. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22933" in your Materials and Methods section.