pcDNA FLAG TORC2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22975||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression
Growth in Bacteria
SpeciesM. musculus (mouse)
Insert Size (bp)2000
Entrez GeneCRTC2 (a.k.a. TORC-2, TORC2)
/ Fusion Protein
- FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
EcoRI/NotI cloning sites. Not I cloning site was added to pcDNA 3.0 MCS. Contains stop codon in cDNA sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA FLAG TORC2 was a gift from Marc Montminy (Addgene plasmid # 22975 ; http://n2t.net/addgene:22975 ; RRID:Addgene_22975)
For your References section:TORCs: transducers of regulated CREB activity. Conkright MD, Canettieri G, Screaton R, Guzman E, Miraglia L, Hogenesch JB, Montminy M. Mol Cell. 2003 Aug . 12(2):413-23. 10.1016/j.molcel.2003.08.013 PubMed 14536081