pcDNA3.1 FLAG mTOR 1968-2549 S2035T
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||23000||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression
Growth in Bacteria
SpeciesH. sapiens (human)
MutationS2035T aa 1968-2549 only
Entrez GeneMTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
/ Fusion Protein
- FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site KpnI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1 FLAG mTOR 1968-2549 S2035T was a gift from Jing Huang (Addgene plasmid # 23000 ; http://n2t.net/addgene:23000 ; RRID:Addgene_23000)
For your References section:Target identification using drug affinity responsive target stability (DARTS). Lomenick B, Hao R, Jonai N, Chin RM, Aghajan M, Warburton S, Wang J, Wu RP, Gomez F, Loo JA, Wohlschlegel JA, Vondriska TM, Pelletier J, Herschman HR, Clardy J, Clarke CF, Huang J. Proc Natl Acad Sci U S A. 2009 Dec 7. ():. 10.1073/pnas.0910040106 PubMed 19995983