Addgene - pEM1 = flp-21::LoxPStopLoxP::npr-1 SL2 GFP Plasmid Data

Plasmid 24033: pEM1 = flp-21::LoxPStopLoxP::npr-1 SL2 GFP

Gene/insert name:
Npr1
Species:
C. elegans (nematode)
Entrez Gene:
npr-1 (CELE_C39E6.6)
Fusion protein or tag:
GFP
Terminal:
C terminal on insert
Vector backbone:
flp21::loxPStopLoxP
(Search Vector Database)
Vector type:
Worm Expression, Cre/Lox
Backbone size w/o insert (bp):
10000
Cloning site 5':
NheI
Site destroyed during cloning:
Unknown
Cloning site 3':
KpnI
Site destroyed during cloning:
Unknown
5' sequencing primer:
flp-21 promoter List of Sequencing Primers
3' sequencing primer:
GFP-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Cori Bargmann
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A hub-and-spoke circuit drives pheromone attraction and social behaviour in C. elegans. Macosko et al (Nature. 2009 Apr 30. 458(7242):1171-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24033" in your Materials and Methods section.