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pLVU/GFP
(Plasmid #24177)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24177 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti6/UbC/V5-Dest
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 9998
  • Vector type
    Mammalian Expression, Lentiviral ; Gateway
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdB survival strain
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Chloramphenicol resistance gene (CmR) - ccdB gene
  • Species
    Chloramphenicol resistance gene (CmR) - ccdB gene
  • Tag / Fusion Protein
    • eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR1 (destroyed during cloning)
  • 3′ cloning site attR2 (destroyed during cloning)
  • 5′ sequencing primer sequence
  • 3′ sequencing primer sequence
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

V5 tag deleted

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVU/GFP was a gift from Lars Ittner (Addgene plasmid # 24177 ; http://n2t.net/addgene:24177 ; RRID:Addgene_24177)
  • For your References section:

    Gateway-compatible lentiviral transfer vectors for ubiquitin promoter driven expression of fluorescent fusion proteins. Krupka N, Strappe P, Götz J, Ittner LM. Plasmid. 2010 Feb 2. ():. 10.1016/j.plasmid.2010.01.002 PubMed 20132838