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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 24217 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3945
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepGint
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Alt nameGFP splicing reporter
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SpeciesModified from Aequorea victoria and adenovirus
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Insert Size (bp)859
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MutationModified adenovirus in GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer commercial, from Clontech (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGint was a gift from Mariano Garcia-Blanco (Addgene plasmid # 24217 ; http://n2t.net/addgene:24217 ; RRID:Addgene_24217) -
For your References section:
A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice. Bonano VI, Oltean S, Garcia-Blanco MA. Nat Protoc. 2007 . 2(9):2166-81. 10.1038/nprot.2007.292 PubMed 17853873