pGint
(Plasmid #24217)

• Depositing Lab: Mariano Garcia-Blanco
• Publication: Bonano et al Nat Protoc. 2007 . 2(9):2166-81.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	24217	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pEGFP-N1
  (Search Vector Database)
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3945
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pGint
• Alt name: GFP splicing reporter
• Species: Modified from Aequorea victoria and adenovirus
• Insert Size (bp): 859
• Mutation: Modified adenovirus in GFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: commercial, from Clontech

Resource Information

• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 4 References