pGint
(Plasmid #24217)

• Depositing Lab: Mariano Garcia-Blanco
• Publication: Bonano et al Nat Protoc. 2007 . 2(9):2166-81.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3945
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pGint
• Alt name: GFP splicing reporter
• Species: Modified from Aequorea victoria and adenovirus
• Insert Size (bp): 859
• Mutation: Modified adenovirus in GFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: commercial, from Clontech

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry