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PLCe EF-C
(Plasmid #244968)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244968 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pCMV
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 8500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    phospholipase C epsilon
  • Alt name
    PLCepsilon
  • Alt name
    PLC epsilon
  • Alt name
    PLCe
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    3735
  • Mutation
    N-terminal truncation that removes residues 1-1036.
  • Entrez Gene
    Plce1 (a.k.a. Plce)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site Kpn1 (not destroyed)
  • 5′ sequencing primer 5'-CGCAAATGGGCGGTAGGCGTG-3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original vector encoding full-length PLCe was a gift from Alan V. Smrcka (U. Michigan) in 2013.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.14.623250 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PLCe EF-C was a gift from Angeline Lyon (Addgene plasmid # 244968)

  • For your References section:

    RhoA Allosterically Activates Phospholipase Cepsilon via its EF Hands. Ohri V, Samassekou K, Muralidharan K, Garland-Kuntz EE, Fisher IJ, Hogan WC, Davis BM, Lyon AM. bioRxiv [Preprint]. 2025 Jun 17:2024.11.14.623250. doi: 10.1101/2024.11.14.623250. 10.1101/2024.11.14.623250 PubMed 39605621
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