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pCMV/SB10ΔDDE
(Plasmid #24552)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24552 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMVbeta
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3712
  • Modifications to backbone
    pCMV/SB10 (Addgene plasmid # 24551) was cut with MscI to remove 322 bp of the transposase coding region which deleted much of the catalytic DDE domain and disrupted the reading frame by introducing a premature translational termination codon.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sleeping Beauty delta DDE
  • Alt name
    SBdDDE
  • Alt name
    SB10dDDE
  • Insert Size (bp)
    698
  • Mutation
    removes 322 bp of the transposase-coding region
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (destroyed during cloning)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer M13 Forward
  • 3′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV/SB10ΔDDE was a gift from Perry Hackett (Addgene plasmid # 24552 ; http://n2t.net/addgene:24552 ; RRID:Addgene_24552)
  • For your References section:

    Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. Ivics Z, Hackett PB, Plasterk RH, Izsvak Z. Cell. 1997 Nov 14. 91(4):501-10. 10.1016/S0092-8674(00)80436-5 PubMed 9390559