Addgene - pFloxin-IRES-Ofd1-Myc-A80T Plasmid Data

Plasmid 24575: pFloxin-IRES-Ofd1-Myc-A80T

Gene/insert name:
Ofd1
Insert size:
3087
Species:
M. musculus (mouse)
GenBank ID:
NM_177429
Entrez Gene:
Ofd1 (ORF2, Cxorf5, DXGgc7e)
Fusion protein or tag:
Myc
Terminal:
C terminal on insert
Mutation:
Alanine 80 changed to threonine (codon GCA to ACA)
Vector backbone:
pBluescript
(Search Vector Database)
Vector type:
Mouse Targeting, Cre/Lox
Backbone size w/o insert (bp):
4873
5' sequencing primer:
tcggtgcacatgctttacat List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl2
Growth temperature (℃):
37
Growth instructions:
Stbl2 (Invitrogen) or other RecA- strain 37 degrees in LB media
High or low copy:
Low Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Jeremy Reiter
Terms and Licenses:
MTA

Comments:

Mutation reported in:

Four novel mutations in the OFD1 (Cxorf5) gene in Finnish patients with oral-facial-digital syndrome 1.

Rakkolainen A, Ala-Mello S, Kristo P, Orpana A, Järvelä I.

J Med Genet. 2002 Apr;39(4):292-6. No abstract available. PMID: 11950863

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Ofd1, a human disease gene, regulates the length and distal structure of centrioles. Singla et al (Dev Cell. 2010 Mar 16. 18(3):410-24. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24575" in your Materials and Methods section.

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